An improved method for isolation of RNA from bone

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An improved method for isolation of RNA from bone

BACKGROUND Bone physiology is increasingly appreciated as an important contributor to metabolic disorders such as type 2 diabetes. However, progress in understanding the role of bone in determining metabolic health is hampered by the well-described difficulty of obtaining high quality RNA from bone for gene expression analysis using the currently available approaches. RESULTS We developed a s...

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Isolation of RNA from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. Although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different typ...

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An Improved Method of RNA Isolation from Loblolly Pine (P. taeda L.) and Other Conifer Species

Tissues isolated from conifer species, particularly those belonging to the Pinaceae family, such as loblolly pine (Pinus taeda L.), contain high concentrations of phenolic compounds and polysaccharides that interfere with RNA purification. Isolation of high-quality RNA from these species requires rigorous tissue collection procedures in the field and the employment of an RNA isolation protocol ...

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Running head: RNA isolation from adult maritime pine needles Article type: Commentary Title: An improved method for high quality RNA isolation from needles of adult maritime pine trees

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ژورنال

عنوان ژورنال: BMC Biotechnology

سال: 2012

ISSN: 1472-6750

DOI: 10.1186/1472-6750-12-5